Nakamura N, Hashimoto K, Matsunaga T
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Anal Chem. 1991 Feb 1;63(3):268-72. doi: 10.1021/ac00003a015.
We have developed a novel immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.
我们开发了一种使用细菌磁性颗粒测定免疫球蛋白G（IgG）的新型免疫测定方法。制备了异硫氰酸荧光素（FITC）偶联的抗IgG细菌磁性颗粒。使用荧光分光光度计测量由FITC-抗IgG抗体-细菌磁性颗粒缀合物的凝集引起的荧光猝灭。基于特异性免疫反应的聚集体通过明胶溶液分离。通过施加磁场增强细菌磁性颗粒缀合物的聚集。相对荧光强度与0.5-100 ng/mL范围内的IgG浓度呈线性相关。
