Nargessi R D, Pourfarzaneh M, Landon J
Clin Chim Acta. 1981 Mar 19;111(1):65-8. doi: 10.1016/0009-8981(81)90422-8.
A simple fluoroimmunoassay is described for the determination of immunoglobulin G (IgG) in serum. It employs IgG labelled with fluorescein and magnetisable polyacrolein/iron oxide particles covalently linked to goat anti-human IgG serum. Diluted patient sample or standard and fluorescein-labelled IgG are incubated for 30 min with solid-phase antibody, the antibody-bound and free antigen separated simply by means of a magnet and the fluorescence in the supernate (free fraction) determined, which directly reflects the IgG concentration of the standard or sample. Correlation studies with two other immunological methods showed good agreement.
本文描述了一种用于测定血清中免疫球蛋白G（IgG）的简单荧光免疫测定法。它采用用荧光素标记的IgG和与山羊抗人IgG血清共价连接的可磁化聚丙烯醛/氧化铁颗粒。将稀释的患者样本或标准品与荧光素标记的IgG与固相抗体孵育30分钟，通过磁铁简单分离结合抗体和游离抗原，然后测定上清液（游离部分）中的荧光，这直接反映了标准品或样本的IgG浓度。与其他两种免疫学方法的相关性研究显示出良好的一致性。
