Zhang Li-tian, Yao Yong-ming, Lu Jia-qi, Yu Yan
Burns Institute, First Hospital Affiliated to the Chinese People's Liberation Army General Hospital, Beijing 100037, China.
Zhonghua Wai Ke Za Zhi. 2007 Oct 1;45(19):1342-5.
To investigate changes in endogenous bactericidal/permeability-increasing protein (BPI) levels and their significance in patients with surgical sepsis.
In the prospective study, 19 surgical patients with infection were involved. The plasma BPI, lipopolysaccharide-binding protein (LBP) and interleukin-6 levels were measured on post-infected days 1, 3, 5, 7 and 14 by an enzyme-linked immunosorbent assay (ELISA). Plasma endotoxin concentrations were determined by the modified chromogenic Limulus Amebocyte Lysate (LAL).
Compared with normal controls, significant lower plasma BPI/LBP ratios were observed in septic patients on days 1 to 5 after infection (P < 0.01), and in severe septic patients on days 1 to 7 (P < 0.01). Moreover, plasma BPI/LBP ratios were much lower in severe sepsis than those in sepsis on days 1 to 3 after infection (P < 0.05).
Plasma BPI and LBP levels increased rapidly after infection, but BPI/LBP ratios were significantly decreased during sepsis. Plasma BPI/LBP ratios appear to be closely related to the severity of sepsis in patients complicated by surgical infection.
探讨外科脓毒症患者内源性杀菌/通透性增加蛋白（BPI）水平的变化及其意义。
在这项前瞻性研究中，纳入了19例感染的外科患者。采用酶联免疫吸附测定（ELISA）法在感染后第1、3、5、7和14天检测血浆BPI、脂多糖结合蛋白（LBP）和白细胞介素-6水平。采用改良显色鲎试剂法测定血浆内毒素浓度。
与正常对照组相比，脓毒症患者在感染后第1至5天（P<0.01）以及严重脓毒症患者在感染后第1至7天（P<0.01）的血浆BPI/LBP比值显著降低。此外，在感染后第1至3天，严重脓毒症患者的血浆BPI/LBP比值明显低于脓毒症患者（P<0.05）。
感染后血浆BPI和LBP水平迅速升高，但脓毒症期间BPI/LBP比值显著降低。血浆BPI/LBP比值似乎与外科感染并发脓毒症患者的严重程度密切相关。
