Pu W, Yourong L
Zhejiang Institute of Technology, Hangzhou, China.
Chin J Biotechnol. 1991;7(4):309-18.
The biosynthesis of glucose oxidase (GOD) by Penicillium-producing strain Z-I-C in batch and chemostat culture was studied. In a glucose-limited chemostat, the experimental results showed that the maintenance coefficient of this strain was 0.04 g glucose/g dry cell wt/h; yield factor of growth was 0.714 g dry cell wt/g glucose; maximum specific growth rate was 0.385 h and saturation constant Ks was 4.76 g/l. Theoretical optimum dilution rate was 0.260 h-1. Maximum specific enzyme activity was increased from 1.51 x 10(3) mu/mg in batch culture to 2.16 x 10(3) mu/mg in chemostat culture and to 3.11 x 10(3) mu/mg in chemostat culture supplemented in feed medium with 0.02% inducer, alpha-methyl-D-glucose, a 43% or 106% increase, respectively, compared with that of batch culture.
研究了产青霉素菌株Z-I-C在分批培养和恒化器培养中葡萄糖氧化酶（GOD）的生物合成。在葡萄糖限制的恒化器中，实验结果表明该菌株的维持系数为0.04 g葡萄糖/ g干细胞重/ h；生长得率系数为0.714 g干细胞重/ g葡萄糖；最大比生长速率为0.385 h，饱和常数Ks为4.76 g / l。理论最佳稀释率为0.260 h-1。最大比酶活从分批培养中的1.51×10（3）μ/ mg增加到恒化器培养中的2.16×10（3）μ/ mg，并在补料培养基中添加0.02％诱导剂α-甲基-D-葡萄糖的恒化器培养中增加到3.11×10（3）μ/ mg，与分批培养相比分别增加了43％或106％。
