Wiedey J, Alexander M Sipski, Marson L
Department of Surgery, Division of Urology, School of Medicine, 2335 MBRB, CB 7052, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7052, USA.
Brain Res. 2008 Mar 4;1197:106-14. doi: 10.1016/j.brainres.2007.12.071. Epub 2008 Jan 12.
The overlapping distribution of spinal neurons activated with either pudendal sensory nerve or pelvic nerve stimulation was examined in the female rat using c-fos immunohistochemistry. Pudendal sensory nerve stimulation resulted in a significant increase in fos-positive cells in the ipsilateral dorsal horn and bilaterally in the medial, lateral and intermediate gray of L5-S1. Pelvic nerve stimulation resulted in significant increases of c-fos immunoreactive nuclei in the ipsilateral dorsal horn, lateral and intermediate gray and bilaterally in the medial gray of L5-S1. Co-distribution of fos immunoreactive nuclei with the vesicular glutamate transporters (VGlut2 and VGlut3) and neurokinin I receptors were found in distinct regions of the dorsal horn, medial and lateral gray. Specific areas in the medial dorsal horn, dorsal gray commissure, laminae VI and X and dorsal lateral gray were activated after stimulation of the pudendal sensory and pelvic nerves, suggesting these areas contain spinal neurons that receive both somatomotor and visceral inputs and are part of the intraspinal circuit that regulates sexual and voiding function.
利用c-fos免疫组织化学方法，在雌性大鼠中研究了阴部感觉神经或盆神经刺激所激活的脊髓神经元的重叠分布情况。阴部感觉神经刺激导致同侧背角以及L5-S1节段双侧的内侧、外侧和中间灰质中fos阳性细胞显著增加。盆神经刺激导致同侧背角、外侧和中间灰质以及L5-S1节段双侧内侧灰质中c-fos免疫反应性细胞核显著增加。在背角、内侧和外侧灰质的不同区域发现了fos免疫反应性细胞核与囊泡谷氨酸转运体（VGlut2和VGlut3）以及神经激肽I受体的共同分布。阴部感觉神经和盆神经刺激后，内侧背角、背侧灰质连合、VI层和X层以及背外侧灰质中的特定区域被激活，表明这些区域包含接受躯体运动和内脏输入的脊髓神经元，并且是调节性和排尿功能的脊髓内回路的一部分。
