Ostrowski J, Dołowy K, Zych W, Butruk E
Department of Gastroenterology and Metabolism, Medical Center of Postgraduate Education, Warszawa, Poland.
J Physiol Pharmacol. 1991 Dec;42(4):367-79.
The present studies were designed to examine the effect of changes in membrane potential by means of protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) and variations in the pH of the medium on the secretory response of parietal cells. Studies were performed in vitro using isolated cells from rat stomachs and acid production was indirectly determined by 14C-aminopyrine (AP) accumulation. CCCP affected both basal and histamine-stimulated AP accumulation in a concentration-dependent manner. The AP accumulation ratios depended on pH of the incubation medium; the ratio was lowest at pH 6.6, and increased progressively as the pH of the medium increased to 7.8. Moreover, the decreases in AP accumulation ratios caused by simultaneous addition of CCCP and AP to cell suspensions compared to those in which CCCP was added to incubated cells after achieving the steady-state of AP accumulation were quantitatively similar. These findings suggest that the decrease in AP accumulation due to CCCP treatment is a consequence of an activation of acid secretion rather than an inhibitor of acid production. From the present and previously published data, we propose a working hypothesis: membrane recycling is dependent on changes in apical membrane potential.
本研究旨在通过质子载体羰基氰化物间氯苯腙（CCCP）改变膜电位以及改变培养基pH值，来检测其对壁细胞分泌反应的影响。研究采用体外实验，使用从大鼠胃中分离出的细胞，通过14C-氨基比林（AP）蓄积间接测定酸分泌量。CCCP以浓度依赖的方式影响基础和组胺刺激的AP蓄积。AP蓄积率取决于孵育培养基的pH值；该比率在pH 6.6时最低，并且随着培养基pH值增加到7.8而逐渐升高。此外，与在AP蓄积达到稳态后将CCCP添加到孵育细胞中相比，同时向细胞悬液中添加CCCP和AP所导致的AP蓄积率降低在数量上相似。这些发现表明，CCCP处理导致的AP蓄积减少是酸分泌激活的结果，而非酸产生的抑制剂所致。根据目前及先前发表的数据，我们提出一个工作假说：膜循环依赖于顶端膜电位的变化。
