Zhao Ru-Song, Wang Xia, Yuan Jin-Peng, Lin Jin-Ming
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, China.
J Chromatogr A. 2008 Mar 7;1183(1-2):15-20. doi: 10.1016/j.chroma.2008.01.021. Epub 2008 Jan 16.
This paper demonstrates, for the first time, that adsorptive potential of bamboo charcoal for solid-phase extraction of phthalate esters was investigated. The four phthalate esters, dimethyl phthalate (DMP), diethyl phthalate (DEP), butyl benzyl phthalate (BBP) and di-n-butyl phthalate (DBP), are quantitatively adsorbed on a bamboo charcoal packed cartridge, then the analytes retained on the cartridge are quantitatively desorbed with optimum amounts of acetone. Finally, the analytes in the eluant acetone are determined by high-performance liquid chromatography-ultraviolet detectior. Important parameters influencing the extraction efficiency, such as eluant and its volume, flow rate of sample, sample volume, pH, the amount of adsorbent and ionic strength were investigated and optimized in detail. Under the optimum conditions, the limits of detection were 0.35-0.43 microg/L for four phthalate esters. The proposed method has been applied to the analysis of rainwater and tap water samples. And satisfactory spiked recoveries were obtained in the range of 75.0-114.2%. All the results indicated that the bamboo charcoal has great potential as a novel adsorbent material for the enrichment and determination of phthalate esters in real environmental water samples.
本文首次展示了对竹炭用于邻苯二甲酸酯固相萃取的吸附潜力进行的研究。四种邻苯二甲酸酯，即邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸丁苄酯（BBP）和邻苯二甲酸二正丁酯（DBP），被定量吸附在装有竹炭的柱上，然后用适量的丙酮将保留在柱上的分析物定量解吸。最后，通过高效液相色谱 - 紫外检测器测定洗脱液丙酮中的分析物。详细研究并优化了影响萃取效率的重要参数，如洗脱液及其体积、样品流速、样品体积、pH、吸附剂用量和离子强度。在最佳条件下，四种邻苯二甲酸酯的检测限为0.35 - 0.43μg/L。该方法已应用于雨水和自来水样品的分析，加标回收率在75.0 - 114.2%范围内，结果令人满意。所有结果表明，竹炭作为一种新型吸附材料，在实际环境水样中邻苯二甲酸酯的富集和测定方面具有巨大潜力。
