Roussakis Emmanuel, Voutsadaki Styliani, Pinakoulaki Eftychia, Sideris Dionisia P, Tokatlidis Kostas, Katerinopoulos Haralambos E
Department of Chemistry, University of Crete, Heraklion 71003, Crete, Greece.
Cell Calcium. 2008 Sep;44(3):270-5. doi: 10.1016/j.ceca.2007.12.008. Epub 2008 Feb 19.
A new fluorescent Zn2+ indicator, namely, ICPBCZin was synthesized and the spectral profile of its free and Zn2+ bound forms was studied. The newly synthesized zinc indicator incorporates as chromophore the chromeno [3',2':3,4]pyrido[1,2a] [1,3]benzimidazole moiety and belongs to the dicarboxylate-type of zinc probes. The compound is excited with visible light, exhibits high selectivity for zinc in the presence of calcium and other common biological ions, and its Zn2+ dissociation constant is 4.0 nM. Fluorescence spectra studies of ICPBCZin indicated a clear shift in its emission wavelength maxima upon Zn2+ binding, as it belongs to the class of Photoinduced Charge Transfer (PCT) indicators, along with changes in fluorescence intensity that enable the compound to be used as a ratiometric, visible-excitable Zn2+ probe.
合成了一种新型荧光锌离子指示剂ICPBCZin，并研究了其游离形式和锌离子结合形式的光谱特征。新合成的锌指示剂以苯并色烯[3',2':3,4]吡啶并[1,2a][1,3]苯并咪唑部分作为发色团，属于二羧酸盐型锌探针。该化合物由可见光激发，在钙和其他常见生物离子存在下对锌具有高选择性，其锌离子解离常数为4.0 nM。ICPBCZin的荧光光谱研究表明，在锌离子结合后其发射波长最大值有明显位移，因为它属于光诱导电荷转移（PCT）指示剂类别，同时荧光强度也发生变化，这使得该化合物能够用作比率型、可见光激发的锌离子探针。
