生物体内可动锌成像。
Imaging mobile zinc in biology.
机构信息
Department of Chemistry 18-498, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
出版信息
Curr Opin Chem Biol. 2010 Apr;14(2):225-30. doi: 10.1016/j.cbpa.2009.12.010. Epub 2010 Jan 22.
Abstract
Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence emission properties have provided sensitive tools for mobile zinc imaging, and fluorescence microscopy experiments have afforded depictions of zinc distribution within live cells and tissues. Both small-molecule and protein-based fluorescent probes can address complex imaging challenges, such as analyte quantification, site-specific sensor localization, and real-time detection.
摘要
锌离子在细胞内外的运输和调节影响着包括大脑、胰腺和前列腺在内的多个器官的细胞功能和病理状态。为了更深入地了解锌离子在活细胞中的分布和动态变化，人们开发了越来越复杂的探针。基于荧光发射性质变化来响应锌离子结合的检测系统为研究细胞内锌离子的分布提供了灵敏的工具，荧光显微镜实验则能够描绘活细胞和组织内的锌离子分布。小分子和蛋白基荧光探针可以解决复杂的成像挑战，如分析物定量、特定位置传感器定位和实时检测。
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