Schröder Ewald, Eaton Philip
King's College London, Department of Cardiology, Cardiovascular Division, The Rayne Institute, St. Thomas' Hospital, London SE1 7EH, UK.
Curr Opin Pharmacol. 2008 Apr;8(2):153-9. doi: 10.1016/j.coph.2007.12.012. Epub 2008 Feb 19.
Exogenous H(2)O(2) is widely applied to cardiovascular tissues in order to elicit oxidant-dependent responses relevant to signalling and disease. Lower levels of endogenous H(2)O(2) are essential for normal physiological functioning and signalling, whereas higher levels are associated with disease. Within diseased tissues, concentrations in excess of 100 microM have been measured, though 1-15 microM appears to be the upper limit of the healthy physiological range. Analysing the kinetic constants and abundance of peroxidases suggests that they may, on occasion, encounter tissue H(2)O(2) concentrations as high as 1 mM. Extracellular application of 0.01-1 mM peroxide appears to be directly relevant to biology and broadly mimics the release of H(2)O(2) endogenously by growth factors and other effectors. However, the intracellular H(2)O(2) may only ever reach 1-15% of the applied exogenous concentration.
外源性过氧化氢广泛应用于心血管组织，以引发与信号传导和疾病相关的氧化依赖性反应。较低水平的内源性过氧化氢对于正常生理功能和信号传导至关重要，而较高水平则与疾病相关。在患病组织中，已测得浓度超过100微摩尔/升，尽管1 - 15微摩尔/升似乎是健康生理范围的上限。分析过氧化物酶的动力学常数和丰度表明，它们有时可能会遇到高达1毫摩尔/升的组织过氧化氢浓度。细胞外施加0.01 - 1毫摩尔/升的过氧化物似乎与生物学直接相关，并广泛模拟生长因子和其他效应物内源性释放的过氧化氢。然而，细胞内过氧化氢可能仅达到所施加外源性浓度的1 - 15%。
