Glover M G, Hargens A R, Mahmood M M, Gott S, Brown M D, Garfin S R
Division of Orthopaedics and Rehabilitation, University of California, San Diego.
J Orthop Res. 1991 Jan;9(1):61-7. doi: 10.1002/jor.1100090109.
Swelling of the intervertebral disc nucleus pulposus may be a contributing factor in lower back pain syndromes. We have designed and tested a new osmometer for in vitro determination of nucleus pulposus swelling pressure. The functional principle of the osmometer involves compressing a sample of nucleus pulposus with nitrogen gas until saline pressure gradients across a 0.45-micron Millipore filter are eliminated. Swelling pressures of both pooled dog and pooled pig lumbar disc nucleus pulposus were measured on the new osmometer and were compared with swelling pressure determined using the equilibrium dialysis technique. The osmometer measured swelling pressures comparable to those obtained by the dialysis technique. This osmometer provides a rapid, direct, and accurate measurement of swelling pressure of the nucleus pulposus.
椎间盘髓核肿胀可能是下背部疼痛综合征的一个促成因素。我们设计并测试了一种新的渗透压计，用于体外测定髓核肿胀压力。该渗透压计的工作原理是用氮气压缩髓核样本，直到消除0.45微米微孔滤膜上的盐液压差。在新的渗透压计上测量了混合的狗和猪腰椎间盘髓核的肿胀压力，并与使用平衡透析技术测定的肿胀压力进行了比较。该渗透压计测得的肿胀压力与透析技术测得的相当。这种渗透压计能快速、直接且准确地测量髓核的肿胀压力。
