Iwabuchi C, Iwabuchi K, Kobayashi S, Ogasawara K, Negishi I, Wang B Y, Wambua P P, Arase H, Fukushi N, Itoh Y
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
J Immunol. 1991 Jan 1;146(1):26-34.
Allogeneic bone marrow chimeras were prepared using reciprocal combinations of AKR and C3H mice. When C3H mice were recipients, the number of thymocytes recoverable from such chimeras (C3H recipient chimeras) was small as compared with that from chimeras for which AKR mice were used as recipients (AKR recipient chimeras) regardless of donor strain. The thymocytes from C3H recipient chimeras showed a profound deficiency in generating proliferative responses to stimulation by anti-CD3 mAb (2C11) or anti-TCR (alpha, beta) mAb (H57-597), even though the expression of CD3 and TCR molecules fell within the same range as that in AKR recipient chimeras. Furthermore, after stimulation with immobilized 2C11, the proportion of IL-2R+ cells in the thymocytes from C3H recipient chimeras was much less than that in AKR recipient chimeras. However, no significant difference in proliferative responses to 2C11 plus PMA, in influx of Ca2+ after stimulation with 2C11 or IL-2 production in response to 2C11 plus PMA or PMA plus A23187 was demonstrated between C3H and AKR recipient chimeras. These findings suggest that the thymocytes from C3H recipient chimeras have a deficiency in the signal transduction system as compared with chimeras for which AKR mice are the recipients. The thymic stromal component involved in this difference in the C3H recipient chimeras is discussed.
使用AKR和C3H小鼠的相互组合制备了同种异体骨髓嵌合体。当C3H小鼠作为受体时，与以AKR小鼠作为受体的嵌合体（AKR受体嵌合体）相比，无论供体品系如何，从此类嵌合体（C3H受体嵌合体）中可回收的胸腺细胞数量都很少。C3H受体嵌合体的胸腺细胞在产生对抗CD3单克隆抗体（2C11）或抗TCR（α，β）单克隆抗体（H57-597）刺激的增殖反应方面存在严重缺陷，尽管CD3和TCR分子的表达与AKR受体嵌合体中的表达范围相同。此外，在用固定化的2C11刺激后，C3H受体嵌合体的胸腺细胞中IL-2R+细胞的比例远低于AKR受体嵌合体。然而，在C3H和AKR受体嵌合体之间，未显示出对2C11加PMA的增殖反应、用2C11刺激后的Ca2+内流或对2C11加PMA或PMA加A23187的IL-2产生有显著差异。这些发现表明，与以AKR小鼠作为受体的嵌合体相比，C3H受体嵌合体的胸腺细胞在信号转导系统方面存在缺陷。本文讨论了参与C3H受体嵌合体这种差异的胸腺基质成分。
