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具有哺育活性的胸腺基质细胞克隆在体外支持小鼠CD4+8+胸腺细胞的生长和分化。

Thymic stromal cell clone with nursing activity supports the growth and differentiation of murine CD4+8+ thymocytes in vitro.

作者信息

Nishimura T, Takeuchi Y, Ichimura Y, Gao X H, Akatsuka A, Tamaoki N, Yagita H, Okumura K, Habu S

机构信息

Department of Immunology, Tokai University School of Medicine, Isehara, Japan.

出版信息

J Immunol. 1990 Dec 15;145(12):4012-7.

PMID:1979583
Abstract

Thymic stromal cell clone, TNC-R3.1 cell, was established from spontaneous AKR/J mouse thymoma. TNC-R3.1 cell, which has the similar properties to thymic nurse cells, formed a unique complex with normal thymocyte subpopulations. Flow cytometry analysis demonstrated that CD4+8+ and CD4-8- immature thymocytes preferentially interacted with TNC-R3.1 stromal cell clone. CD4+8+ thymocytes, which interacted with TNC-R3.1 stromal cell clone, contained a higher proportion of large size and cycling T cells than did noninteracting CD4+8+ thymocytes. As is generally accepted, CD4+8+ thymocytes did not respond to any stimulation such as IL-2, anti-CD3 mAb (2C11), or IL-2 plus 2C11. However, culture of isolated CD4+8+ thymocytes on TNC-R3.1 stromal cell monolayer in the presence of suboptimal dose of IL-2 induced a significant cell growth. Moreover, the addition of 2C11 and IL-2 into this coculture system resulted in a dramatic increase of the proliferative response of thymocytes. Flow cytometry analysis showed the proliferating cells on TNC-R3.1, which originated from CD4+8+ thymocytes, were mostly TCR-alpha beta+ CD3+CD4-8+ T cells. These results provide in vitro evidence that CD4+8+ thymocytes are at an intermediate stage of T cell maturation and TNC-R3.1 stromal cell clone induces the growth and differentiation of CD4+8+ thymocytes into CD4-8+ T cells.

摘要

胸腺基质细胞克隆TNC-R3.1细胞是从自发的AKR/J小鼠胸腺瘤中建立的。TNC-R3.1细胞具有与胸腺哺育细胞相似的特性，能与正常胸腺细胞亚群形成独特的复合物。流式细胞术分析表明，CD4+8+和CD4-8-未成熟胸腺细胞优先与TNC-R3.1基质细胞克隆相互作用。与TNC-R3.1基质细胞克隆相互作用的CD4+8+胸腺细胞中，大尺寸和处于细胞周期的T细胞比例高于未相互作用的CD4+8+胸腺细胞。通常认为，CD4+8+胸腺细胞对任何刺激如白细胞介素-2、抗CD3单克隆抗体（2C11）或白细胞介素-2加2C11均无反应。然而，在次优剂量白细胞介素-2存在的情况下，将分离的CD4+8+胸腺细胞培养在TNC-R3.1基质细胞单层上可诱导显著的细胞生长。此外，在此共培养系统中加入2C11和白细胞介素-2会导致胸腺细胞增殖反应急剧增加。流式细胞术分析显示，在TNC-R3.1上增殖的细胞源自CD4+8+胸腺细胞，大多为TCR-αβ+CD3+CD4-8+T细胞。这些结果提供了体外证据，表明CD4+8+胸腺细胞处于T细胞成熟的中间阶段，且TNC-R3.1基质细胞克隆可诱导CD4+8+胸腺细胞生长并分化为CD4-8+T细胞。

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