通过基因组改组筛选和培育高产紫杉醇真菌
Screening and breeding of high taxol producing fungi by genome shuffling.
作者信息
Zhao Kai, Ping WenXiang, Zhang LiNa, Liu Jun, Lin Yan, Jin Tao, Zhou DongPo
机构信息
Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, 150080, China.
出版信息
Sci China C Life Sci. 2008 Mar;51(3):222-31. doi: 10.1007/s11427-008-0037-5.
To apply the fundamental principles of genome shuffling in breeding of taxol-producing fungi, Nodulisporium sylviform was used as starting strain in this work. The procedures of protoplast fusion and genome shuffling were studied. Three hereditarily stable strains with high taxol production were obtained by four cycles of genome shuffling. The qualitative and quantitative analysis of taxol produced was confirmed using thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and LC-MS. A high taxol producing fungus, Nodulisporium sylviform F4-26, was obtained, which produced 516.37 microg/L taxol. This value is 64.41% higher than that of the starting strain NCEU-1 and 31.52%-44.72% higher than that of the parent strains.
为了将基因组改组的基本原理应用于产紫杉醇真菌的育种，本研究以拟盘多毛孢属真菌作为出发菌株。对原生质体融合和基因组改组的程序进行了研究。通过四轮基因组改组获得了三株遗传稳定且紫杉醇产量高的菌株。利用薄层色谱法（TLC）、高效液相色谱法（HPLC）和液相色谱-质谱联用（LC-MS）对所产紫杉醇进行了定性和定量分析。获得了一株高产紫杉醇的真菌，即拟盘多毛孢属真菌F4-26，其紫杉醇产量为516.37μg/L。该产量比出发菌株NCEU-1高64.41%，比亲本菌株高31.52%-44.72%。
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