Gu Yu, Wang Yanlin, Ma Xiaoping, Wang Chengdong, Yue Guizhou, Zhang Yuetian, Zhang Yunyan, Li Shanshan, Ling Shanshan, Liu Xiaomin, Wen Xintian, Cao Sanjie, Huang Xiaobo, Deng Junliang, Zuo Zhicai, Yu Shumin, Shen Liuhong, Wu Rui
College of Life Sciences, Sichuan Agricultural University, Ya'an, 625014, China.
Appl Biochem Biotechnol. 2015 Jan;175(1):155-65. doi: 10.1007/s12010-014-1254-y. Epub 2014 Sep 23.
While taxol yields of fungi from non-animal sources are still low, whether Pestalotiopsis hainanensis isolated from the scurf of a dermatitic giant panda, Ailuropoda melanoleuca, provides a greater taxol yield remains unknown. The objective of the study was to determine the corresponding taxol yield. The structure of the taxol produced by the fungus was evaluated by thin layer chromatography (TLC), ultraviolet (UV) spectroscopy, high-performance liquid chromatography (HPLC), (1)H and (13)C nuclear magnetic resonance spectroscopy ((1)H-NMR and (13)C-NMR), and time-of-flight mass spectrometry (TOF-MS), with standard taxol as a control. The results demonstrated that the P. hainanensis fungus produced taxol, which had the same structure as the standard taxol and yield of 1,466.87 μg/L. This fungal taxol yield from the dermatitic giant panda was significantly greater than those of fungus from non-animal sources. The taxol-producing fungus may be a potential candidate for the production of taxol on an industrial scale.
虽然非动物来源真菌的紫杉醇产量仍然很低，但从患有皮肤病的大熊猫（Ailuropoda melanoleuca）皮屑中分离出的海南盘多毛孢（Pestalotiopsis hainanensis）是否能产生更高的紫杉醇产量仍不清楚。该研究的目的是确定相应的紫杉醇产量。以标准紫杉醇为对照，通过薄层色谱法（TLC）、紫外光谱法（UV）、高效液相色谱法（HPLC）、氢核磁共振光谱法（¹H-NMR）、碳核磁共振光谱法（¹³C-NMR）和飞行时间质谱法（TOF-MS）对该真菌产生的紫杉醇结构进行了评估。结果表明，海南盘多毛孢真菌产生了紫杉醇，其结构与标准紫杉醇相同，产量为1466.87μg/L。这种来自患有皮肤病大熊猫的真菌紫杉醇产量显著高于非动物来源真菌。产紫杉醇的真菌可能是工业规模生产紫杉醇的潜在候选者。
