Socher S H, Krall J F, Jaffe R C, O'Malley B W
Endocrinology. 1976 Sep;99(3):891-900. doi: 10.1210/endo-99-3-891.
Chromatin from the oviducts of estrogen-treated chicks was sheared and fractionated on sucrose gradients. This resulted in the production of several chromatin fractions which differ in their sedimentation properties, protein composition, the number of acceptor sites for the progesterone-receptor complex, and the ability to serve as a template for RNA synthesis. The pellet chromatin fraction shows an enhanced ability to bind the progesterone-receptor complex in vitro and in cell-free systems. Kinetic analysis indicates that the majority of the acceptor sites for the progesterone-receptor complex are located in the pellet chromatin fraction which is lowest in template activity. The sites may be important loci for initiating the changes in RNA synthesis following the exposure of target cells to steroid hormones.
对经雌激素处理的雏鸡输卵管染色质进行剪切，并在蔗糖梯度上进行分级分离。这产生了几种染色质组分，它们在沉降特性、蛋白质组成、孕酮受体复合物的受体位点数量以及作为RNA合成模板的能力方面存在差异。沉淀染色质组分在体外和无细胞系统中显示出更强的结合孕酮受体复合物的能力。动力学分析表明，孕酮受体复合物的大多数受体位点位于模板活性最低的沉淀染色质组分中。这些位点可能是靶细胞暴露于类固醇激素后启动RNA合成变化的重要位点。
