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鉴定与含有5'剪接位点的RNA特异性结合的核蛋白。

Identification of nuclear proteins that specifically bind to RNAs containing 5' splice sites.

作者信息

Stolow D T, Berget S M

机构信息

Marrs McClean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.

出版信息

Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):320-4. doi: 10.1073/pnas.88.2.320.

DOI:10.1073/pnas.88.2.320
PMID:1824871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC50802/
Abstract

Two polypeptides of 26 and 37 kDa (designated SPP-1 and SPP-2) were identified in in vitro splicing extracts by UV crosslinking to splicing precursor RNAs. Crosslinking of both polypeptides required a functional 5' splice site but was not dependent on sequences at the 3' end of the intron. Centrifugation of extract separated the two polypeptides from major U small nuclear ribonucleoproteins (snRNPs), including U1 snRNPs. Both polypeptides crosslinked to precursor RNAs containing 5' splice sites in the absence of U1 RNA. Complexes containing both polypeptides also contained U1 snRNPs, suggesting that SPP-1 and SPP-2 are a part of the functional spliceosome. We propose that SPP-1 and SPP-2 are factors that participate in the recognition of 5' splice sites.

摘要

通过与剪接前体RNA进行紫外光交联，在体外剪接提取物中鉴定出了两条分子量分别为26 kDa和37 kDa的多肽（分别命名为SPP-1和SPP-2）。两条多肽的交联都需要一个功能性的5'剪接位点，但不依赖于内含子3'端的序列。提取物的离心分离将这两条多肽与包括U1 snRNP在内的主要U小核核糖核蛋白（snRNP）分离开来。在没有U1 RNA的情况下，两条多肽都与含有5'剪接位点的前体RNA发生交联。包含这两条多肽的复合物中也含有U1 snRNP，这表明SPP-1和SPP-2是功能性剪接体的一部分。我们提出，SPP-1和SPP-2是参与5'剪接位点识别的因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/3c2b0f2cd938/pnas01052-0017-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/44d432a0f03a/pnas01052-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/ddf71cc5dd10/pnas01052-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/cb951396f00e/pnas01052-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/8d4bf32f69b6/pnas01052-0016-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/02e36998df91/pnas01052-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/bbdf520b376a/pnas01052-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/3c2b0f2cd938/pnas01052-0017-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/44d432a0f03a/pnas01052-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/ddf71cc5dd10/pnas01052-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/cb951396f00e/pnas01052-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/8d4bf32f69b6/pnas01052-0016-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/02e36998df91/pnas01052-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/bbdf520b376a/pnas01052-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9be/50802/3c2b0f2cd938/pnas01052-0017-c.jpg

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本文引用的文献

1
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Cell. 1981 Jun;24(3):679-86. doi: 10.1016/0092-8674(81)90094-5.
2
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
3
The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
剪接体组装过程中前体mRNA与六种U2小核核糖核蛋白之间的直接相互作用。
Mol Cell Biol. 1994 May;14(5):2994-3005. doi: 10.1128/mcb.14.5.2994-3005.1994.
4
Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins.异质性核糖核蛋白A1及其他异质性核糖核蛋白A/B蛋白保守结构域的功能。
EMBO J. 1994 Nov 15;13(22):5483-95. doi: 10.1002/j.1460-2075.1994.tb06883.x.
5
Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.通过体内过表达果蝇异质性细胞核核糖核蛋白实现外显子跳跃。
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1822-5. doi: 10.1073/pnas.92.6.1822.
6
The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites.人类剪接因子ASF/SF2能够特异性识别前体mRNA的5'剪接位点。
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3363-7. doi: 10.1073/pnas.91.8.3363.
7
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8
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9
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10
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6
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7
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8
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10
The yeast RNA gene products are essential for mRNA splicing in vitro.酵母RNA基因产物对于体外mRNA剪接至关重要。
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