Manabe Takayuki, Tatsumi Kouko, Inoue Masahide, Matsuyoshi Hiroko, Makinodan Manabu, Yamauchi Takahira, Makinodan Eri, Yokoyama Shohei, Sakumura Ryoko, Okuda Hiroaki, Wanaka Akio
Department of 2nd Anatomy, Faculty of Medicine, Nara Medical University, 840 Shijyo-cho, Kasihara City, Nara 634-8521, Japan.
Int J Dev Neurosci. 2008 Apr;26(2):249-52. doi: 10.1016/j.ijdevneu.2007.11.004. Epub 2007 Dec 4.
L3/Lhx8, a member of the Lim-homeobox gene family, is selectively and specifically expressed in the murine embryonic medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-deficient mice specifically lack cholinergic neurons in the basal forebrain. In this manuscript, we report the in vitro effects of reduced L3/Lhx8 gene expression on cholinergic differentiation in murine embryonic stem (ES) cell-derived spheres without dissociation. The knockdown of L3/Lhx8 gene expression dramatically decreased the cholinergic phenotype of spheres without altering other known phenotypes (TuJ1, GABA and GFAP). These results strongly suggest that L3/Lhx8 is a key factor for cholinergic differentiation of murine ES cell-derived spheres and is involved in basal forebrain development.
L3/Lhx8是Lim-同源框基因家族的成员之一，在小鼠胚胎内侧神经节隆起（MGE）中选择性且特异性地表达。我们之前的研究表明，L3/Lhx8基因缺陷型小鼠的基底前脑特异性缺乏胆碱能神经元。在本论文中，我们报告了L3/Lhx8基因表达降低对未解离的小鼠胚胎干细胞（ES）来源的球状体中胆碱能分化的体外影响。L3/Lhx8基因表达的敲低显著降低了球状体的胆碱能表型，而未改变其他已知表型（TuJ1、GABA和GFAP）。这些结果强烈表明，L3/Lhx8是小鼠ES细胞来源的球状体胆碱能分化的关键因素，并参与基底前脑发育。
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