An orthogonal purification strategy for isolating crosslinked domains of modular synthases.
作者信息
Haushalter Robert W, Worthington Andrew S, Hur Gene H, Burkart Michael D
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0358, USA.
出版信息
Bioorg Med Chem Lett. 2008 May 15;18(10):3039-42. doi: 10.1016/j.bmcl.2008.01.026. Epub 2008 Jan 11.
Abstract
Chemo-enzymatic methods for covalently crosslinking carrier proteins with partner enzymes within modular synthases hold promise for elucidating and engineering metabolic pathways. Our efforts to crystallize the ACP-KS complexes of fatty acid synthases have been complicated by difficulties in the purification of the crosslinked complex from the other proteins in the reaction. Here we present a solution that employs an orthogonal purification strategy to achieve the quantity and level of purity necessary for further studies of this complex.
摘要
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1
Mechanism-based protein cross-linking probes to investigate carrier protein-mediated biosynthesis.
ACS Chem Biol. 2006 Dec 20;1(11):687-91. doi: 10.1021/cb6003965.
2
Synthesis and evaluation of bioorthogonal pantetheine analogues for in vivo protein modification.
J Am Chem Soc. 2006 Sep 20;128(37):12174-84. doi: 10.1021/ja063217n.
3
Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research.
DNA Res. 2005;12(5):291-9. doi: 10.1093/dnares/dsi012. Epub 2006 Jan 9.
4
Architecture of a fungal fatty acid synthase at 5 A resolution.
Science. 2006 Mar 3;311(5765):1263-7. doi: 10.1126/science.1123251.
5
Architecture of mammalian fatty acid synthase at 4.5 A resolution.
Science. 2006 Mar 3;311(5765):1258-62. doi: 10.1126/science.1123248.
6
One-pot chemo-enzymatic synthesis of reporter-modified proteins.
Org Biomol Chem. 2006 Jan 7;4(1):44-6. doi: 10.1039/b512735a. Epub 2005 Nov 24.
7
In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues.
J Am Chem Soc. 2005 Aug 17;127(32):11234-5. doi: 10.1021/ja052911k.
8
Proteome-scale purification of human proteins from bacteria.
Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2654-9. doi: 10.1073/pnas.042684199.
9
Identification and analysis of the acyl carrier protein (ACP) docking site on beta-ketoacyl-ACP synthase III.
J Biol Chem. 2001 Mar 16;276(11):8231-8. doi: 10.1074/jbc.M008042200. Epub 2000 Nov 14.
10
Crystal structure of beta-ketoacyl-acyl carrier protein synthase III. A key condensing enzyme in bacterial fatty acid biosynthesis.
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