Ugolini Luisa, De Nicola Gina, Palmieri Sandro
Research Center for Industrial Crops, Agriculture Research Council, Via di Corticella 133, I-40128 Bologna, Italy.
J Agric Food Chem. 2008 Mar 12;56(5):1595-601. doi: 10.1021/jf072582a. Epub 2008 Feb 6.
Cruciferous oilseeds are important sources of oil, proteins, and glucosinolates (GLs), potentially available when biorefinery processes are used. The proposed extraction technology is based on the use of reverse micelles (RMs) made with cetyltrimethylammonium bromide (CTAB) dispersed in organic solvent. The physicochemical properties of this extraction system and the good water solubility of many high value compounds, such as GLs and some proteins, permit the simultaneous extraction of oil, and these products from cruciferous oilseed meals. This procedure is based on three main steps: (i) seed conditioning; (ii) solid-liquid extraction by RM solution; and (iii) back-transfer of the RM solution for recovery of the extracted compounds. The method makes it possible to simultaneously extract almost the same amount of oil as with pure organic solvents used in the current extraction plants and more than 90% of soluble proteins and GLs. It is a promising biorefinery technology alternative to traditional oil extraction processes.
十字花科油料种子是油脂、蛋白质和硫代葡萄糖苷（GLs）的重要来源，在采用生物精炼工艺时可能会获取这些成分。所提出的提取技术基于使用由十六烷基三甲基溴化铵（CTAB）制成的反胶束（RMs），其分散于有机溶剂中。该提取系统的物理化学性质以及许多高价值化合物（如硫代葡萄糖苷和一些蛋白质）的良好水溶性，使得能够从十字花科油料种子粕中同时提取油脂和这些产品。此过程基于三个主要步骤：（i）种子预处理；（ii）用反胶束溶液进行固液萃取；（iii）反胶束溶液的逆向转移以回收萃取的化合物。该方法能够同时提取几乎与当前提取工厂中使用的纯有机溶剂相同量的油脂，以及超过90%的可溶性蛋白质和硫代葡萄糖苷。它是一种有前景的生物精炼技术，可替代传统的油脂提取工艺。
