Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China; School of Life Sciences, Huizhou University, Huizhou 516007, China.
Food Chem. 2019 Dec 1;300:125162. doi: 10.1016/j.foodchem.2019.125162. Epub 2019 Jul 9.
Moringa oleifera is a worldwide cultivated edible and medicinal plant. Its seeds are rich in oil, proteins, and glucosinolates. A practical method was developed to simultaneously extract and separate the three groups of substances from M. oleifera seeds. Smashed seed material was loaded into columns with petroleum ether: ethanol 8:2 (PE-ethanol) and eluted sequentially with 4.8-fold PE-ethanol to extract oil, and 10.8-fold water to extract proteins and glucosinolates. More than 95% of oil, proteins, and glucosinolates were extracted. The extracts were separated automatically into ether (oil) phase and ethanol aqueous phase. The latter was further separated into proteins and glucosinolates by 70% ethanol precipitation. The main glucosinolate was identified by LC-MS as GLC (4-α-rhamnopyranosyloxy-benzyl glucosinolate). After purification, 22.3 g refined oil, 33.0 g proteins, and 5.5 g purified GLC from 100 g M. oleifera seeds were obtained. This study provides a simple and high-efficient method to utilize M. oleifera seeds.
辣木是一种在世界范围内广泛种植的食用和药用植物。它的种子富含油、蛋白质和硫代葡萄糖苷。本文开发了一种实用的方法，可从辣木种子中同时提取和分离这三组物质。将粉碎的种子材料装入填充有石油醚:乙醇 8:2（PE-乙醇）的柱子中，然后依次用 4.8 倍的 PE-乙醇洗脱以提取油，用 10.8 倍的水提取蛋白质和硫代葡萄糖苷。超过 95%的油、蛋白质和硫代葡萄糖苷被提取。提取物自动分离成乙醚（油）相和乙醇水相。后者通过 70%乙醇沉淀进一步分离为蛋白质和硫代葡萄糖苷。通过 LC-MS 鉴定主要的硫代葡萄糖苷为 GLC（4-α-鼠李吡喃糖氧基-苄基硫代葡萄糖苷）。从 100g 辣木种子中得到了 22.3g 精炼油、33.0g 蛋白质和 5.5g 纯化的 GLC。本研究提供了一种简单高效的利用辣木种子的方法。
