Gomes Carolina C, Drummond Sergio N, Guimarães André L S, Andrade Carlos I, Mesquita Ricardo A, Gomez Ricardo S
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
J Oral Pathol Med. 2008 Mar;37(3):151-6. doi: 10.1111/j.1600-0714.2007.00604.x.
Genetic factors are known to be involved in oral squamous cell carcinoma (OSCC) development.
We evaluated a possible association between CCND1 A870G and P21/WAF1 C98A polymorphisms and OSCC, as well as the impact of the genotypes on protein immunoexpression. The study group consisted of 80 individuals with histopathological diagnosis of OSCC and the control group consisted of 80 healthy individuals without oral lesions and matched by age, sex and tobacco usage. The genotypes were studied by the polymerase chain reaction and restriction fragment length polymorphic analysis. Paraffin-embedded sections were used for immunohistochemical analysis.
No statistical association between CCND1 and/or P21/WAF1 genotypes and OSCC was demonstrated, although we found that people harbouring the combined presence of at least one variant allele of both genes showed a 1.8 times more chance of developing OSCC compared to the referent genotype. OSCC tumours from individuals with P21 heterozygous genotype showed a significantly higher immunopositivity than tumours from wild-type individuals.
The present study did not demonstrate a significant association between CCND1 and / or P21 / WAF1 genotypes and OSCC. However, P21 protein expression in OSCC tumours is affected by P21 / WAF1 genotype.
已知遗传因素参与口腔鳞状细胞癌（OSCC）的发生发展。
我们评估了CCND1 A870G和P21/WAF1 C98A基因多态性与OSCC之间的可能关联，以及这些基因型对蛋白质免疫表达的影响。研究组由80例经组织病理学诊断为OSCC的个体组成，对照组由80例无口腔病变的健康个体组成，两组在年龄、性别和烟草使用方面相匹配。通过聚合酶链反应和限制性片段长度多态性分析研究基因型。石蜡包埋切片用于免疫组织化学分析。
未证实CCND1和/或P21/WAF1基因型与OSCC之间存在统计学关联，尽管我们发现同时携带两个基因至少一个变异等位基因的个体患OSCC的几率是参照基因型个体的1.8倍。P21杂合基因型个体的OSCC肿瘤显示出比野生型个体的肿瘤显著更高的免疫阳性率。
本研究未证实CCND1和/或P21/WAF1基因型与OSCC之间存在显著关联。然而，OSCC肿瘤中的P21蛋白表达受P21/WAF1基因型影响。
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