Wang M, Van Duijn B, Schram A W
Centre for Phytotechnology RUL/TNO, Department of Molecular Plant Biotechnology, Leiden, The Netherlands.
FEBS Lett. 1991 Jan 14;278(1):69-74. doi: 10.1016/0014-5793(91)80086-i.
Cytosolic calcium concentrations (Cai) of barley aleurone protoplasts after stimulation with the plant hormone abscisic acid (ABA) were measured by using the calcium-sensitive fluorescent dye Indo-1. The measured basal Cai is about 200 nM. Stimulation with ABA induces a strong dose-dependent decrease in Cai to a minimal value of about 50 nM. This decrease occurs within 5 s. The Ca2+ antagonists La3+ and Cd2+ inhibit the ABA-induced Cai decrease in a dose-dependent manner, while the Ca2+ channel blockers verapamil and nifedipine give no inhibition. The induction of Cai decrease by ABA is consistent with activation of the plasma membrane Ca2(+)-ATPase by ABA. The possible role of this ABA-induced Cai decrease in ABA signal transduction and in counteracting the effects of gibberellic acid are discussed.
利用钙敏感荧光染料吲哚-1测量了植物激素脱落酸（ABA）刺激后大麦糊粉层原生质体的胞质钙浓度（Cai）。测得的基础Cai约为200 nM。ABA刺激会导致Cai强烈的剂量依赖性降低，降至约50 nM的最小值。这种降低在5秒内发生。Ca2+拮抗剂La3+和Cd2+以剂量依赖性方式抑制ABA诱导的Cai降低，而Ca2+通道阻滞剂维拉帕米和硝苯地平则无抑制作用。ABA诱导的Cai降低与ABA对质膜Ca2(+)-ATP酶的激活一致。讨论了这种ABA诱导的Cai降低在ABA信号转导以及对抗赤霉素作用中的可能作用。
