Zafiropoulos G G, Flores-de-Jacoby L, Todt G, Kolb G, Havemann K, Tatakis D N
Department of Periodontology, Phillipps University of Marburg, F.R.G.
J Periodontal Res. 1991 Jan;26(1):24-32. doi: 10.1111/j.1600-0765.1991.tb01622.x.
This investigation analyzed, in a cross-sectional study, the possible relationship between gingival crevicular fluid (GCF) elastase-like protease (ELP) levels and the periodontal clinical parameters or the presence of specific bacteria in subgingival plaque. A total of 388 periodontal sites from 8 adult periodontitis patients were examined for plaque index (PII), gingival index (GI), pocket depth (PD) and alveolar bone loss (ABL). GCF ELP levels were determined as ELP alpha-1 protease inhibitor (ELP-alpha 1-PI) complex levels with a commercially available ELISA. Subgingival plaque samples were tested for the presence of Bacteroides gingivalis, B. intermedius and Actinobacillus actinomycetemcomitans by indirect immunofluorescence (IF) microscopy. GCF ELP-alpha 1-PI levels were then correlated with clinical periodontal indices and proportions of IF-positive bacteria per site. Statistically significant positive correlations were found between GCF ELP-alpha 1-PI concentrations and subgingival Bacteroides proportions. When the sites examined were analyzed depending on the level of each clinical parameter, the levels of these correlations changed. A. actinomycetemcomitans correlated highly (r = 0.716) with ABL for sites with low GI score. The correlations between GCF ELP-alpha 1-PI and B. gingivalis (r = 0.642) or B. intermedius (r = 0.774) were the highest for ABL less than or equal to 20% and PD less than or equal to 3 mm, respectively. The strong association between GCF ELP-alpha 1-PI concentrations and subgingival bacteria previously associated with advancing periodontitis indicates that measurement of GCF ELP-alpha 1-PI concentrations may be useful in the evaluation of periodontal sites, especially those with very little or no tissue destruction.
在一项横断面研究中，本调查分析了龈沟液（GCF）弹性蛋白酶样蛋白酶（ELP）水平与牙周临床参数或龈下菌斑中特定细菌的存在之间的可能关系。对8例成人牙周炎患者的388个牙周部位进行了菌斑指数（PII）、牙龈指数（GI）、牙周袋深度（PD）和牙槽骨吸收（ABL）检查。采用市售酶联免疫吸附测定（ELISA）法将GCF ELP水平测定为ELPα1蛋白酶抑制剂（ELP-α1-PI）复合物水平。通过间接免疫荧光（IF）显微镜检测龈下菌斑样本中牙龈拟杆菌、中间普氏菌和伴放线放线杆菌的存在情况。然后将GCF ELP-α1-PI水平与临床牙周指数以及每个部位IF阳性细菌的比例进行关联分析。发现GCF ELP-α1-PI浓度与龈下拟杆菌比例之间存在统计学上显著的正相关。根据每个临床参数的水平对检查部位进行分析时，这些相关性的水平发生了变化。对于GI评分低的部位，伴放线放线杆菌与ABL高度相关（r = 0.716）。对于ABL小于或等于20%和PD小于或等于3 mm的部位，GCF ELP-α1-PI与牙龈拟杆菌（r = 0.642）或中间普氏菌（r = 0.774）之间的相关性分别最高。GCF ELP-α1-PI浓度与先前与进展性牙周炎相关的龈下细菌之间的强关联表明，测量GCF ELP-α1-PI浓度可能有助于评估牙周部位，尤其是那些组织破坏很少或没有组织破坏的部位。
