Harper D S, Lamster I B, Celenti R
Fairleigh Dickinson University Oral Health Research Center, Hackensack, NJ 07601.
J Clin Periodontol. 1989 Mar;16(3):164-9. doi: 10.1111/j.1600-051x.1989.tb01634.x.
Examining the relationships among indicators of the acute inflammatory response in gingival crevicular fluid (GCF) and specific bacterial species in subgingival plaque may provide indications of which bacterial species or groups of species may be associated with potentially destructive host-derived processes. Here we report on the relationship of the subgingival plaque flora to the activity of mammalian forms of the enzymes beta-glucuronidase (beta G), lactate dehydrogenase (LDH), and arylsulfatase (AS) in GCF from a total of 54 4-6 mm periodontal sites from 13 periodontitis patients. Sites were scored for probing depth (PD) and bleeding on probing, and GCF was collected using filter paper strips inserted into the sulcus for 30 s, eluted in buffer and assayed for enzyme activity. 1 week later, the patients were again evaluated for PD and bleeding, and subgingival plaque was removed with a curette oriented toward the pocket epithelium. Plaque samples were examined by darkfield microscopy and cultured anaerobically on selective and non-selective media. Various groups of bacteria, including species of black pigmenting Bacteroides (BPB), Fusobacterium sp., Capnocytophaga sp, Streptococcus sanguis, and total facultative organisms were enumerated. Relationships among the enzymes and bacterial groups expressed as colony-forming unit (CFU) counts or as a % of the total cultivable flora were assessed by Spearman correlation analysis. beta G levels were significantly correlated with populations of spirochetes, B. intermedius, B. gingivalis, and total lactose negative BPB's. Correlation between beta G and F. nucleatum sp. or Capnocytophaga sp. approached but did not reach statistically significant levels. In contrast, LDH activity showed a significant positive correlation with levels of B. gingivalis and total lactose negative BPB's. AS levels were significantly correlated only with B. gingivalis. beta G and LDH showed a significant negative correlation with levels of coccoid forms. Thus, beta G, an acid hydrolase which can serve as a marker for primary granule release from polymorphonuclear leukocytes, was most closely correlated with the micro-organisms found in other studies to be associated with chronic adult periodontitis.
研究龈沟液（GCF）中急性炎症反应指标与龈下菌斑中特定细菌种类之间的关系，可能会提示哪些细菌种类或菌群可能与潜在的具有破坏性的宿主衍生过程相关。在此，我们报告了来自13名牙周炎患者的总共54个4 - 6毫米牙周位点的龈下菌斑菌群与GCF中哺乳动物形式的β-葡萄糖醛酸酶（βG）、乳酸脱氢酶（LDH）和芳基硫酸酯酶（AS）活性之间的关系。对位点进行探诊深度（PD）和探诊出血评分，使用插入龈沟30秒的滤纸条收集GCF，在缓冲液中洗脱并测定酶活性。1周后，再次对患者进行PD和出血评估，并用刮匙朝向袋上皮去除龈下菌斑。通过暗视野显微镜检查菌斑样本，并在选择性和非选择性培养基上进行厌氧培养。对包括产黑色素拟杆菌（BPB）、梭杆菌属、二氧化碳嗜纤维菌属、血链球菌以及总兼性菌等各类细菌进行计数。通过Spearman相关分析评估酶与以菌落形成单位（CFU）计数或占可培养菌群总数百分比表示的细菌群之间的关系。βG水平与螺旋体、中间拟杆菌、牙龈拟杆菌以及总乳糖阴性BPB的数量显著相关。βG与具核梭杆菌或二氧化碳嗜纤维菌之间的相关性接近但未达到统计学显著水平。相比之下，LDH活性与牙龈拟杆菌和总乳糖阴性BPB的水平呈显著正相关。AS水平仅与牙龈拟杆菌显著相关。βG和LDH与球菌形式的水平呈显著负相关。因此，βG作为一种酸性水解酶，可作为多形核白细胞初级颗粒释放的标志物，与其他研究中发现的与慢性成人牙周炎相关的微生物关系最为密切。
