Warren H S, Skipsey L J
Cancer Research Unit, Royal Canberra Hospital South, Woden ACT, Australia.
Immunology. 1991 Jan;72(1):150-7.
A subpopulation of human peripheral blood natural killer (NK) cells, defined by sedimentation at Percoll high buoyant densities (P greater than 1.0635-1.0640 g/ml) and unresponsiveness to interleukin-2 (IL-2), contained two distinct populations based on the intensity of CD16 (FcR gamma III) expression, namely CD16dim and CD16bright. This resting subpopulation of NK cells differed from the total population of peripheral blood NK cells, by containing a larger proportion of CD16dim cells, by the total absence of CD56bright CD16- cells, and by an inability to respond to high concentrations (500 U/ml) of rIL-2 despite the expression of an intermediate affinity (p70) IL-2R. Both CD16dim and CD16bright NK cells expressing high affinity IL-2R were initially generated following co-culture of resting NK cells with gamma-irradiated MM-170 cells and IL-2, but CD16bright NK cells became the dominant cell type later in culture. The CD16 molecule was not involved in the differentiation of resting NK cells since solid-phase-bound anti-CD16 monoclonal antibody neither enhanced nor inhibited NK cell generation. These studies demonstrate that the resting subpopulation of peripheral blood NK cells expresses a unique CD16 profile, that CD16 expression increases during NK cell generation, and that CD16 is not involved in the differentiation process.
人类外周血自然杀伤（NK）细胞的一个亚群，通过在Percoll高浮力密度（P大于1.0635 - 1.0640 g/ml）下的沉降以及对白细胞介素-2（IL-2）无反应来定义，根据CD16（FcRγIII）表达强度包含两个不同的群体，即CD16dim和CD16bright。这个静息NK细胞亚群与外周血NK细胞的总体不同，在于含有更大比例的CD16dim细胞，完全不存在CD56bright CD16-细胞，并且尽管表达中等亲和力（p70）的IL-2R，但无法对高浓度（500 U/ml）的重组IL-2作出反应。表达高亲和力IL-2R的CD16dim和CD16bright NK细胞最初是在静息NK细胞与γ射线照射的MM - 170细胞及IL-2共培养后产生的，但在培养后期CD16bright NK细胞成为主要细胞类型。CD16分子不参与静息NK细胞的分化，因为固相结合的抗CD16单克隆抗体既不增强也不抑制NK细胞的产生。这些研究表明，外周血NK细胞的静息亚群表达独特的CD16谱型，CD16表达在NK细胞产生过程中增加，并且CD16不参与分化过程。
