London L, Perussia B, Trinchieri G
J Immunol. 1985 Feb;134(2):718-27.
In this report, we show that resting human peripheral blood NK cells, positively enriched with antibody B73.1, can be induced to proliferate in vitro in short-term cultures, and newly express membrane activation antigens. B73.1+ NK cells, cultured for 6 days in the presence of conditioned medium containing IL 2 and irradiated B lymphoblastoid cells, show significant [3H]TdR incorporation beginning on day 4. At that time, a large proportion of the cells express HLA-DR and 4F2 antigens, and transferrin and IL 2 receptors, all detectable at high density by indirect immunofluorescence with the use of monoclonal antibodies. These cells maintain their ability to lyse target cells spontaneously or in the presence of antibodies. By two-color immunofluorescence and cell cycle analysis combined with indirect immunofluorescence, we demonstrate directly that the activation antigens are expressed on all NK (B73.1+) cells in the S/G2/M phases of the cell cycle, and on only a proportion of those in the G0/G1 phases.
在本报告中，我们表明，经抗体B73.1阳性富集的静息人外周血NK细胞，在短期培养中可被诱导在体外增殖，并新表达膜激活抗原。在含有IL-2和经辐照的B淋巴母细胞的条件培养基存在下培养6天的B73.1 + NK细胞，从第4天开始显示出显著的[3H]TdR掺入。此时，很大一部分细胞表达HLA-DR和4F2抗原，以及转铁蛋白和IL-2受体，使用单克隆抗体通过间接免疫荧光均可在高密度下检测到。这些细胞保持了自发或在抗体存在下裂解靶细胞的能力。通过双色免疫荧光和细胞周期分析结合间接免疫荧光，我们直接证明激活抗原在细胞周期的S/G2/M期的所有NK（B73.1 +）细胞上表达，而在G0/G1期的细胞中只有一部分表达。
