Simonetti Oriana, Cirioni Oscar, Goteri Gaia, Ghiselli Roberto, Kamysz Wojciech, Kamysz Elzbieta, Silvestri Carmela, Orlando Fiorenza, Barucca Claudia, Scalise Alessandro, Saba Vittorio, Scalise Giorgio, Giacometti Andrea, Offidani Annamaria
Dermatological Clinic, Università Politecnica delle Marche, Ancona, Italy.
Peptides. 2008 Apr;29(4):520-8. doi: 10.1016/j.peptides.2007.12.011. Epub 2008 Jan 3.
We investigated the effect of topical temporin A in the management of methicillin-resistant strain of Staphylococcus aureus (MRSA)-infected experimental surgical wounds in mice. The wound, cut through the panniculus carnosus of BALB/c mice, was inoculated with 5x10(7) colony-forming units of MRSA. Mice were treated with Allevyn, temporin A-soaked Allevyn, Allevyn and daily intraperitoneal teicoplanin (7mg/kg), temporin A-soaked Allevyn and daily intraperitoneal teicoplanin. Main outcome measurements were: quantitative bacterial culture, histological examination with assessment of micro-vessel density and of vascular endothelial growth factor (VEGF) expression in tissue sections, and VEGF plasma levels alike. Treatment with temporin-A associated with teicoplanin injection significantly reduced bacterial load to 0.85 x 10(1)+/-0.1 x 10(1)CFU/ml. Histological examination showed that infected mice receiving temporin A-soaked Allevyn (with or without teicoplanin) had a higher degree of granulation tissue formation and collagen deposition compared to the other treated groups. A significant increase in serum VEGF expression was observed in mice receiving temporin A topically and temporin A topically associated with intraperitoneal teicoplanin. In conclusion our results demonstrated that temporin A is effective in the management of infected wounds, by a significant bacterial growth inhibition and acceleration of wound repair process.
我们研究了局部应用天蚕素A对小鼠耐甲氧西林金黄色葡萄球菌（MRSA）感染的实验性手术伤口的治疗效果。通过切开BALB/c小鼠的肉膜制造伤口，并接种5×10⁷个MRSA菌落形成单位。小鼠分别接受以下处理：使用爱立肤敷料、浸泡过天蚕素A的爱立肤敷料、爱立肤敷料并每日腹腔注射替考拉宁（7mg/kg）、浸泡过天蚕素A的爱立肤敷料并每日腹腔注射替考拉宁。主要观察指标包括：定量细菌培养、组织学检查以评估组织切片中的微血管密度和血管内皮生长因子（VEGF）表达，以及VEGF血浆水平。天蚕素A联合替考拉宁注射治疗可将细菌载量显著降低至0.85×10¹±0.1×10¹CFU/ml。组织学检查显示，与其他治疗组相比，接受浸泡过天蚕素A的爱立肤敷料（无论是否联合替考拉宁）的感染小鼠有更高程度的肉芽组织形成和胶原蛋白沉积。在局部应用天蚕素A以及局部应用天蚕素A联合腹腔注射替考拉宁的小鼠中，观察到血清VEGF表达显著增加。总之，我们的结果表明，天蚕素A通过显著抑制细菌生长和加速伤口修复过程，对感染伤口的治疗有效。
