Osorio Karen M, Lee Song Eun, McDermitt David J, Waghmare Sanjeev K, Zhang Ying V, Woo Hyun Nyun, Tumbar Tudorita
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA.
Development. 2008 Mar;135(6):1059-68. doi: 10.1242/dev.012799. Epub 2008 Feb 6.
Aml1/Runx1 controls developmental aspects of several tissues, is a master regulator of blood stem cells, and plays a role in leukemia. However, it is unclear whether it functions in tissue stem cells other than blood. Here, we have investigated the role of Runx1 in mouse hair follicle stem cells by conditional ablation in epithelial cells. Runx1 disruption affects hair follicle stem cell activation, but not their maintenance, proliferation or differentiation potential. Adult mutant mice exhibit impaired de novo production of hair shafts and all temporary hair cell lineages, owing to a prolonged quiescent phase of the first hair cycle. The lag of stem cell activity is reversed by skin injury. Our work suggests a degree of functional overlap in Runx1 regulation of blood and hair follicle stem cells at an equivalent time point in the development of these two tissues.
Aml1/Runx1控制多个组织的发育过程，是血液干细胞的主要调节因子，并在白血病中发挥作用。然而，尚不清楚它是否在除血液之外的组织干细胞中发挥作用。在此，我们通过上皮细胞中的条件性消融研究了Runx1在小鼠毛囊干细胞中的作用。Runx1的破坏影响毛囊干细胞的激活，但不影响其维持、增殖或分化潜能。成年突变小鼠由于第一个毛发周期的静止期延长，毛干和所有临时毛细胞谱系的从头产生受损。皮肤损伤可逆转干细胞活性的滞后。我们的工作表明，在这两种组织发育的等效时间点，Runx1对血液和毛囊干细胞的调节存在一定程度的功能重叠。
