Brück Wolfram M, Sennett Susan H, Pomponi Shirley A, Willenz Philippe, McCarthy Peter J
Center for Ocean Exploration, Harbor Branch Oceanographic Institution, Fort Pierce, FL 34946, USA.
ISME J. 2008 Mar;2(3):335-9. doi: 10.1038/ismej.2007.91. Epub 2008 Feb 7.
The lithistid sponge Discodermia dissoluta (family Theonellidae), is found in deep-waters throughout the Caribbean sea and is the source of discodermolide, a natural product with potential anticancer properties, and other secondary metabolites. As with other sponges, large numbers of microbes are harbored in the sponge mesohyl. The microbial population of the sponge mesohyl shows an abundance of large filamentous microbes. Fractionation of the dissociated sponge allowed enrichment of this microbe, which was then identified by analysis of the 16S rRNA genes. Its identity was confirmed through the use of fluorescent in situ hybridization. These studies have allowed the identification of this eubacterial microbe as belonging to the genus Entotheonella.
石海绵纲的溶解盘皮海绵（Theonellidae科）分布于加勒比海的深水区，是盘皮海绵内酯（一种具有潜在抗癌特性的天然产物）及其他次生代谢产物的来源。与其他海绵一样，海绵中胶层中栖息着大量微生物。海绵中胶层的微生物群落显示出大量大型丝状微生物。对解离后的海绵进行分级分离可富集这种微生物，然后通过分析16S rRNA基因对其进行鉴定。通过荧光原位杂交技术确认了其身份。这些研究已确定这种真细菌微生物属于Entotheonella属。
