全血血清的中空纤维流场-流分馏法

Hollow-fiber flow field-flow fractionation of whole blood serum.

作者信息

Zattoni Andrea, Rambaldi Diana Cristina, Roda Barbara, Parisi Daniela, Roda Aldo, Moon Myeong Hee, Reschiglian Pierluigi

机构信息

Department of Chemistry G Ciamician, University of Bologna, Via Selmi 2, Bologna, Italy.

出版信息

J Chromatogr A. 2008 Mar 7;1183(1-2):135-42. doi: 10.1016/j.chroma.2008.01.022. Epub 2008 Jan 16.

DOI:10.1016/j.chroma.2008.01.022

PMID:18258247

Abstract

Hollow-fiber flow field-flow fractionation is here applied to untreated, whole human blood serum. Matrix-assisted, laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) of serum fractions shows mass signals in the <30,000 M(r) range where low-abundance, serum protein components are known to be present, though a membrane of nominal 30,000 Da cutoff was employed for the fractionation device. Using diluted sera spiked with low amounts (0.06-0.1%, w/w) of an artificial mixture constituted the human adrenocorticotropic hormone fragments 18-39 (M(r)=2465.7) and 7-38 (M(r)=3659.2), and of bovine insulin (M(r)=5734), horse cytochrome c (M(r)=12384) and chicken lysozyme (M(r)=14388), a hybrid fractionation/microfiltration mechanism shows to govern the separation of the low-M(r) components.

摘要

中空纤维流场-流分级分离法在此应用于未经处理的全人血清。血清级分的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）显示，在<30,000 M(r)范围内有质量信号，已知低丰度血清蛋白成分存在于此范围，尽管分级分离装置采用的是标称截留分子量为30,000 Da的膜。使用添加了少量（0.06 - 0.1%，w/w）人工混合物的稀释血清，该人工混合物由人促肾上腺皮质激素片段18 - 39（M(r)=2465.7）和7 - 38（M(r)=3659.2）、牛胰岛素（M(r)=5734）、马细胞色素c（M(r)=12384）和鸡溶菌酶（M(r)=14388）组成，一种混合分级分离/微滤机制显示可控制低M(r)成分的分离。