Basile Franco, Kassalainen Galina E, Ratanathanawongs Williams S Kim
Department of Chemistry, Colorado School of Mines, Golden, Colorado 80401, USA.
Anal Chem. 2005 May 1;77(9):3008-12. doi: 10.1021/ac048391d.
A simple interface based on an oscillating capillary nebulizer (OCN) is described for direct deposition of eluate from a thermal field-flow fractionation (ThFFF) system onto a matrix-assisted laser desorption/ionization (MALDI) probe. In this study, the polymer-containing eluent from the ThFFF system was mixed on-line with MALDI matrix solution and deposited directly onto a moving MALDI probe. The result was a continuous sample track representative of the fractionation process. Subsequent off-line MALDI-mass spectrometry analysis was performed in automated and manual modes. Polystyrene samples of broad polydispersity were used to characterize the overall system performance. The OCN interface is easy to build and operate without the use of heaters or high voltages and is compatible with any MALDI probe format.
描述了一种基于振荡毛细管雾化器（OCN）的简单接口，用于将热场流分级（ThFFF）系统的洗脱液直接沉积到基质辅助激光解吸/电离（MALDI）探针上。在本研究中，来自ThFFF系统的含聚合物洗脱液与MALDI基质溶液在线混合，并直接沉积到移动的MALDI探针上。结果得到了代表分级过程的连续样品轨迹。随后以自动和手动模式进行离线MALDI-质谱分析。使用宽多分散性的聚苯乙烯样品来表征整个系统的性能。OCN接口易于构建和操作，无需使用加热器或高电压，并且与任何MALDI探针形式兼容。
