Leal Nilma Cintra, de Araújo Figueiroa Angela Cristina Torres, Cavalcanti Valdelúcia Oliveira, da Silva Soraya Cavalcante, Leal-Balbino Tereza Cristina, de Almeida Alzira Maria Paiva, Hofer Ernesto
Centro de Pesquisas Aggeu Magalhães/fiocruz, Av. Moraes Rego s/n, Campus da UFPE, Cidade Universitária, 50670-420, Recife, PE, Brazil.
Trans R Soc Trop Med Hyg. 2008 Mar;102(3):272-6. doi: 10.1016/j.trstmh.2007.12.008. Epub 2008 Feb 6.
Through a continuous bacteriological monitoring programme carried out by the Health Secretariat of the State of Pernambuco, Brazil, two isolates of Vibrio cholerae O1 El Tor Ogawa were discovered in an endemic area in 2001, during a cholera inactive period, along with six V. cholerae non-O1/non-O139 strains and two Aeromonas veronii biovar sobria strains showing an unusual characteristic of agglutination with O1 antiserum. Between that time and 2005, eight other O1 isolates were found. The virulence genes present in the V. cholerae differed among strains, with only three O1 strains harboring the ctxA gene. The O1 and some non-O1/non-O139 strains displayed identical patterns of amplification of the 16S-23S intergenic spacer region. RAPD of the 10 V. cholerae O1 strains, with the two primers used, revealed heterogeneity. The presence of V. cholerae carrying virulence genes in the aquatic basins examined confirms that they constitute a vibrio reservoir during a cholera inactive period, thus strengthening the argument for a continuous monitoring programme and preventative measures for cholera, mainly in the areas where the supply of drinking water is deficient.
通过巴西伯南布哥州卫生秘书处实施的一项持续细菌学监测计划，2001年在霍乱非活跃期，于一个流行地区发现了两株霍乱弧菌O1 El Tor小川型菌株，同时还发现了6株霍乱弧菌非O1/非O139菌株以及2株维罗纳气单胞菌温和生物变种菌株，这些菌株表现出与O1抗血清凝集的异常特征。在那段时间至2005年期间，又发现了另外8株O1分离株。霍乱弧菌中存在的毒力基因在不同菌株间有所差异，只有3株O1菌株携带ctxA基因。O1菌株和一些非O1/非O139菌株在16S - 23S基因间隔区的扩增模式相同。使用两种引物对10株霍乱弧菌O1菌株进行随机扩增多态性DNA分析，结果显示存在异质性。在所检测的水域中存在携带毒力基因的霍乱弧菌，这证实了它们在霍乱非活跃期构成了一个弧菌储存库，从而强化了持续监测计划以及霍乱预防措施的必要性，主要是在饮用水供应不足的地区。
