Plessers L, Eggermont J A, Wuytack F, Casteels R
Laboratorium voor Fysiologie, Campus Gasthuisberg, Katholicke Universiteit Leuven, Belgium.
J Neurosci. 1991 Mar;11(3):650-6. doi: 10.1523/JNEUROSCI.11-03-00650.1991.
Pig cerebellar Purkinje neurons express a high level of Ca2(+)-transport ATPases in their intracellular Ca2+ stores. This was shown at the mRNA level by Northern blotting and in situ hybridization and at the protein level by Western blotting and immunocytochemistry. The majority of the Ca2(+)-transport ATPases in these neurons belongs to the SERCA2b type (i.e., the Ca2(+)-pump isoform found in most nonmuscle cells). The SERCA2a (cardiac/slow-twitch skeletal/smooth muscle) Ca2(+)-pump isoform is expressed only at very low levels. The main Ca2(+)-pump messenger is 6.0 kilobases long and belongs to a class 4-type processing of SERCA2, which is exclusively confined to the cerebrum and cerebellum. Phospholamban, a regulator of the SERCA2 Ca2(+)-transport ATPase in cardiac/slow-twitch skeletal/smooth muscle, could not be detected in Purkinje neurons.
猪小脑浦肯野神经元在其细胞内钙库中高水平表达钙转运ATP酶。这在mRNA水平通过Northern印迹法和原位杂交得以显示，在蛋白质水平通过Western印迹法和免疫细胞化学得以显示。这些神经元中的大多数钙转运ATP酶属于SERCA2b型（即大多数非肌肉细胞中发现的钙泵亚型）。SERCA2a（心脏/慢肌骨骼肌/平滑肌）钙泵亚型仅以极低水平表达。主要的钙泵信使核糖核酸长6.0千碱基，属于SERCA2的4类加工类型，仅局限于大脑和小脑。在浦肯野神经元中未检测到磷酸受磷蛋白，它是心脏/慢肌骨骼肌/平滑肌中SERCA2钙转运ATP酶的调节剂。
