Kaprielian Z, Campbell A M, Fambrough D M
Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218.
Brain Res Mol Brain Res. 1989 Jul;6(1):55-60. doi: 10.1016/0169-328x(89)90028-4.
The expression of a sarcoplasmic reticulum (SR)-like Ca2+-ATPase was studied in the adult chicken cerebellum. A monoclonal antibody. CaS/C1-IgG, specific for the cardiac/slow-twitch skeletal muscle SR Ca2+-ATPase, was used as a probe of protein expression. An immunoblot analysis showed that CaS/C1-IgG recognized similar size polypeptides in adult chicken heart and cerebellum. CaS/C1-IgG recognized fragments of similar size after limited tryptic digestion of cardiac and cerebellar membranes. A two-dimensional alpha-chymotryptic peptide map analysis demonstrated that the cardiac and cerebellar Ca2+-ATPases were structurally very similar. Immunofluorescence microscopy localized the cerebellar Ca2+-ATPase to Purkinje cell bodies and dendritic trees. These results suggest that the well-known Ca2+ uptake system of skeletal and cardiac muscle SR has a remarkably similar counterpart in some neurons.
在成年鸡小脑中研究了一种肌浆网（SR）样Ca2+ - ATP酶的表达。使用一种对心脏/慢肌型骨骼肌SR Ca2+ - ATP酶具有特异性的单克隆抗体CaS/C1 - IgG作为蛋白质表达的探针。免疫印迹分析表明，CaS/C1 - IgG在成年鸡心脏和小脑中识别出大小相似的多肽。对心脏和小脑膜进行有限的胰蛋白酶消化后，CaS/C1 - IgG识别出大小相似的片段。二维α - 糜蛋白酶肽图谱分析表明，心脏和小脑的Ca2+ - ATP酶在结构上非常相似。免疫荧光显微镜检查将小脑Ca2+ - ATP酶定位到浦肯野细胞体和树突树。这些结果表明，骨骼肌和心肌SR中著名的Ca2+摄取系统在某些神经元中具有非常相似的对应物。
