采用基质辅助激光解吸电离飞行时间串联质谱法测定人血浆中阿巴卡韦、安普那韦、去羟肌苷、依非韦伦、奈韦拉平和司他夫定的浓度。
Determination of abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine concentration in human plasma by MALDI-TOF/TOF.
作者信息
Notari Stefania, Mancone Carmine, Alonzi Tonino, Tripodi Marco, Narciso Pasquale, Ascenzi Paolo
机构信息
Istituto Nazionale per le Malattie Infettive IRCCS Lazzaro Spallanzani, Via Portuense 292, I-00149 Roma, Italy.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 1;863(2):249-57. doi: 10.1016/j.jchromb.2008.01.009. Epub 2008 Jan 16.
The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 x 10(-2)-1.00 pmol/microL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 x 10(-2)-1.00 pmol/microL. The limit of quantification value was 1.00 x 10(-2)pmol/microL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.
自从高效抗逆转录病毒疗法（HAART）成为临床实践中的标准治疗方法以来，对抗逆转录病毒药物的治疗药物监测（TDM）的关注度显著提高。TDM有助于确定适合每位患者的最佳给药方案。在此，我们通过标准加入分析法，应用基质辅助激光解吸电离飞行时间/二级飞行时间（MALDI-TOF/TOF）技术对HIV感染患者血浆中的阿巴卡韦、安普那韦、去羟肌苷、依非韦伦、奈韦拉平和司他夫定进行定量分析。在整个探索的抗HIV浓度范围（1.00×10⁻² - 1.00皮摩尔/微升）内，标准加入法的回归呈线性。绝对回收率在80%至110%之间。通过MALDI-TOF/TOF测定的药物浓度值在1.00×10⁻² - 1.00皮摩尔/微升范围内。阿巴卡韦、安普那韦、去羟肌苷、依非韦伦、奈韦拉平和司他夫定的定量限为1.00×10⁻²皮摩尔/微升。
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