Notari Stefania, Mancone Carmine, Alonzi Tonino, Tripodi Marco, Narciso Pasquale, Ascenzi Paolo
Istituto Nazionale per le Malattie Infettive IRCCS Lazzaro Spallanzani, Via Portuense 292, I-00149 Roma, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 1;863(2):249-57. doi: 10.1016/j.jchromb.2008.01.009. Epub 2008 Jan 16.
The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 x 10(-2)-1.00 pmol/microL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 x 10(-2)-1.00 pmol/microL. The limit of quantification value was 1.00 x 10(-2)pmol/microL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.
自从高效抗逆转录病毒疗法（HAART）成为临床实践中的标准治疗方法以来，对抗逆转录病毒药物的治疗药物监测（TDM）的关注度显著提高。TDM有助于确定适合每位患者的最佳给药方案。在此，我们通过标准加入分析法，应用基质辅助激光解吸电离飞行时间/二级飞行时间（MALDI-TOF/TOF）技术对HIV感染患者血浆中的阿巴卡韦、安普那韦、去羟肌苷、依非韦伦、奈韦拉平和司他夫定进行定量分析。在整个探索的抗HIV浓度范围（1.00×10⁻² - 1.00皮摩尔/微升）内，标准加入法的回归呈线性。绝对回收率在80%至110%之间。通过MALDI-TOF/TOF测定的药物浓度值在1.00×10⁻² - 1.00皮摩尔/微升范围内。阿巴卡韦、安普那韦、去羟肌苷、依非韦伦、奈韦拉平和司他夫定的定量限为1.00×10⁻²皮摩尔/微升。
