采用反相高效液相色谱法同时测定人血浆中HIV核苷类似物逆转录酶抑制剂拉米夫定、去羟肌苷、司他夫定、齐多夫定和阿巴卡韦。
Simultaneous determination of the HIV nucleoside analogue reverse transcriptase inhibitors lamivudine, didanosine, stavudine, zidovudine and abacavir in human plasma by reversed phase high performance liquid chromatography.
作者信息
Verweij-van Wissen C P W G M, Aarnoutse R E, Burger D M
机构信息
Department of Clinical Pharmacy, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):121-9. doi: 10.1016/j.jchromb.2004.11.019.
A reversed phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors (NRTIs) lamivudine, didanosine, stavudine, zidovudine and abacavir in plasma. The method involved solid-phase extraction with Oasis MAX cartridges from plasma, followed by high performance liquid chromatography with a SymmetryShield RP 18 column and ultraviolet detection set at a wavelength of 260 nm. The assay was validated over the concentration range of 0.015-5 mg/l for all five NRTIs. The average accuracies for the assay were 92-102%, inter- and intra-day coefficients of variation (CV) were <2.5% and extraction recoveries were higher than 97%. This method proved to be simple, accurate and precise, and is currently in use in our laboratory for the quantitative analysis of NRTIs in plasma.
建立了一种反相高效液相色谱法，用于同时定量测定血浆中的核苷类逆转录酶抑制剂（NRTIs）拉米夫定、去羟肌苷、司他夫定、齐多夫定和阿巴卡韦。该方法包括用Oasis MAX柱从血浆中进行固相萃取，然后用SymmetryShield RP 18柱进行高效液相色谱分析，并在260 nm波长处进行紫外检测。对所有五种NRTIs在0.015 - 5 mg/l的浓度范围内进行了方法验证。该测定方法的平均准确度为92 - 102%，日内和日间变异系数（CV）<2.5%，萃取回收率高于97%。该方法证明简单、准确且精密，目前在我们实验室用于血浆中NRTIs的定量分析。
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