Burenbaatar Byambaa, Bakheit Mohammed A, Plutzer Judit, Suzuki Naoyoshi, Igarashi Ikuo, Ongerth Jerry, Karanis Panagiotis
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 080-8555, Obihiro, Hokkaido, Japan.
Parasitol Res. 2008 Apr;102(5):901-5. doi: 10.1007/s00436-007-0847-6. Epub 2008 Feb 9.
The presence of Cryptosporidium oocysts in 460 animals (439 cattle, 16 kids, and 5 sheep) of Tuv-aimak Mongolian district was investigated by IFT. Cryptosporidium oocysts were found in 116 (26.4%) cattle. Out of the 116 IFT positive samples, 47 were further purified by IMS, investigated by PCR and 11 were found positive. The species and/or genotypes were determined by nested PCR-RFLP and sequence analysis of a fragment of the SSU rRNA gene. The results indicated the presence of Cryptosporidium andersoni in the sequenced samples and C. bovis in two samples as a common infection. No Cryptosporidium oocysts were found in fecal specimens collected from sheep and goats. The present work reports the first data on Cryptosporidium species in animals from Mongolia. Further studies are necessary to understand the epidemiology and transmission of Cryptosporidium in domestic animals in Mongolia.
通过免疫荧光试验（IFT）对图瓦-艾马克蒙古地区460只动物（439头牛、16只羔羊和5只绵羊）隐孢子虫卵囊的存在情况进行了调查。在116头（26.4%）牛中发现了隐孢子虫卵囊。在116份IFT阳性样本中，47份通过免疫磁珠分离法（IMS）进一步纯化，经聚合酶链反应（PCR）检测，发现11份呈阳性。通过巢式PCR-RFLP和小亚基核糖体RNA（SSU rRNA）基因片段的序列分析确定了其种类和/或基因型。结果表明，在测序样本中存在安氏隐孢子虫，在两个样本中发现牛隐孢子虫为常见感染。从绵羊和山羊采集的粪便样本中未发现隐孢子虫卵囊。本研究报告了蒙古动物隐孢子虫种类的首批数据。有必要进一步开展研究，以了解蒙古家畜中隐孢子虫的流行病学和传播情况。
