台湾饲养动物中隐孢子虫的检测与种类鉴定
Detection and species identification of Cryptosporidium from Taiwan feeding animals.
作者信息
Hsu Bing-Mu, Wun Hau-Yang, Hsu Chih-Li Lilian
机构信息
Department of Earth and Environmental Sciences, National Chung Cheng University, 168, University Road, Min-Hsiung, Chiayi, Taiwan, ROC.
出版信息
J Parasitol. 2008 Feb;94(1):252-6. doi: 10.1645/GE-1135.1.
In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Cryptosporidium spp. oocysts. Ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of Cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. Nucleic acid sequencing of the 18S rRNA gene identified 3 clusters of Cryptosporidium spp. Three Cryptosporidium parvum isolates were from cattle and sheep feces. One Cryptosporidium andersoni isolate was detected from pig feces. The other 2 novel Cryptosporidium genotypes were not similar to any known Cryptosporidium spp. according to the DNA sequences of the 18S rRNA gene.
在本研究中，从台湾畜禽养殖场的40个采样点收集了107份粪便样本，以检测隐孢子虫属卵囊。通过酶联免疫吸附测定分析的107份样本中有10份显示存在隐孢子虫属，其中6份样本通过免疫荧光测定和聚合酶链反应同时得到证实。18S rRNA基因的核酸测序鉴定出3个隐孢子虫属簇。3株微小隐孢子虫分离株来自牛和羊粪便。从猪粪便中检测到1株安氏隐孢子虫分离株。根据18S rRNA基因的DNA序列，另外2种新型隐孢子虫基因型与任何已知的隐孢子虫属均不相似。
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