癌症患者的血小板和微粒混合样本中的组织因子活性增加。
Tissue factor activity is increased in a combined platelet and microparticle sample from cancer patients.
作者信息
Tilley Rachel E, Holscher Todd, Belani Rajesh, Nieva Jorge, Mackman Nigel
机构信息
Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA.
出版信息
Thromb Res. 2008;122(5):604-9. doi: 10.1016/j.thromres.2007.12.023. Epub 2008 Feb 11.
BACKGROUND
Cancer patients have an increased risk of thrombosis. Tissue factor (TF) antigen and TF activity associated with microparticles in plasma are elevated in patients with various types of cancer. Of these two measurements, TF activity is considered superior to TF antigen levels because the activity more closely reflects the ability of TF to initiate coagulation. Recent studies showed that platelets also express TF.
OBJECTIVE
To determine the level of TF activity associated with a combined platelet and microparticle sample from cancer patients (n = 20) and healthy individuals (n = 23).
METHODS
TF activity was measured using a two step chromogenic assay and soluble P-selectin was measured by ELISA in healthy controls and metastatic cancer patients.
RESULTS
We determined the composition of a combined platelet and microparticle sample. The sample consisted of platelets, large microparticles (30-200 nm) and membrane debris. We compared the TF activity of a combined platelet and microparticle sample from cancer patients with that from healthy individuals. We found that TF activity in a combined platelet and microparticle sample from cancer patients was higher than in samples from healthy individuals (21.5+/-12.3 pM (n = 20) versus 8.6+/-6.8 pM (n = 23), mean+/-SD, p < 0.001). Cancer patients also had a higher level of soluble P-selectin compared with controls (18.9+/-5.5 ng/mL versus 13.2+/-2.3 ng/mL, p < 0.001).
CONCLUSION
This study indicates that measurement of TF activity in a combined platelet and microparticle sample can be used as a simple assay to determine the level of circulating TF.
背景
癌症患者发生血栓形成的风险增加。在各类癌症患者中，血浆中与微粒相关的组织因子（TF）抗原及TF活性均升高。在这两种检测指标中，TF活性被认为优于TF抗原水平，因为该活性更能准确反映TF启动凝血的能力。近期研究表明，血小板也表达TF。
目的
测定癌症患者（n = 20）和健康个体（n = 23）的血小板与微粒混合样本中TF的活性水平。
方法
采用两步显色法测定TF活性，通过酶联免疫吸附测定法（ELISA）检测健康对照者和转移性癌症患者的可溶性P选择素。
结果
我们确定了血小板与微粒混合样本的组成。该样本包含血小板、大微粒（30 - 200 nm）及膜碎片。我们比较了癌症患者与健康个体的血小板与微粒混合样本的TF活性。我们发现，癌症患者血小板与微粒混合样本中的TF活性高于健康个体样本（21.5±12.3 pM（n = 20）对8.6±6.8 pM（n = 23），均值±标准差，p < 0.001）。与对照组相比，癌症患者的可溶性P选择素水平也更高（18.9±5.5 ng/mL对13.2±2.3 ng/mL，p < 0.001）。
结论
本研究表明，测定血小板与微粒混合样本中的TF活性可作为一种简单检测方法来确定循环TF水平。
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