Müller K, Maier R
Horm Metab Res. 1976 Jul;8(4):271-3. doi: 10.1055/s-0028-1093633.
The lipolytic activity of ACTH-(1-39) and ACTH-(1-24) in vitro has been compared on a molar basis in assays performed with both adipose-tissue slices and isolated fat cells. In the tissue-slice assay ACTH-(1-24) displayed 29% of the potency of ACTH-(1-39), whereas in the isolated-cell assay, ACTH-(1-24) was 3.9 times more potent than the longer peptide. This discrepancy seems to be due to the fact that ACTH-peptides are inactivated in the adipose-tissue-slice assay, but remain stable in the isolated-fat-cell assay. ACTH-(1-24) is inactivated more rapidly than ACTH-(1-39). Pre-incubation and washing of the tissue slices removes the inactivating principle, which most likely consists of peptidases, and their responsiveness is increased, so that both ACTH-peptides display similar lipolytic activity to that seen in the isolated-cell system. Comparison of the responsiveness of isolated fat cells and isolated adrenal cells indicates that the affinity of ACTH for the receptor of adrenal cells is approximately 100 times greater than its affinity for the fat-cell receptor.
已在脂肪组织切片和分离的脂肪细胞实验中，以摩尔为基础比较了促肾上腺皮质激素（ACTH）-(1 - 39)和促肾上腺皮质激素（ACTH）-(1 - 24)的体外脂解活性。在组织切片实验中，促肾上腺皮质激素（ACTH）-(1 - 24)的效力为促肾上腺皮质激素（ACTH）-(1 - 39)的29%，而在分离细胞实验中，促肾上腺皮质激素（ACTH）-(1 - 24)的效力比更长的肽高3.9倍。这种差异似乎是由于促肾上腺皮质激素肽在脂肪组织切片实验中失活，但在分离脂肪细胞实验中保持稳定。促肾上腺皮质激素（ACTH）-(1 - 24)比促肾上腺皮质激素（ACTH）-(1 - 39)失活更快。对组织切片进行预孵育和洗涤可去除最有可能由肽酶组成的失活因子，其反应性增强，因此两种促肾上腺皮质激素肽均表现出与分离细胞系统中相似的脂解活性。对分离的脂肪细胞和分离的肾上腺细胞反应性的比较表明，促肾上腺皮质激素对肾上腺细胞受体的亲和力比对脂肪细胞受体的亲和力大约高100倍。
