Tarella C, Ferrero D, Bregni M, Siena S, Gallo E, Pileri A, Gianni A M
Dipartimento di Medicina ed Oncologia Sperimentale, Università di Torino, Italy.
Eur J Cancer. 1991;27(1):22-7. doi: 10.1016/0277-5379(91)90052-f.
In 20 patients with non-Hodgkin lymphoma or breast cancer, high-dose cyclophosphamide induced, during the post-nadir period of rapid leucocyte recovery, on median day 19 about a 30-fold increase in the peak concentration of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E) colony-forming cells, and an even higher increase in the more immature pluripotent progenitors (CFU-Mix, 72-fold). After infusion of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), peak concentration was reached earlier (median day 15) and with further enhancements (159, 116 and 283-fold respectively, in the number of CFU-GM, BFU-E and CFU-Mix). Most CFU-GM were immature, lacking the differentiation antigen CD15, and gave rise to large myeloid colonies, reflecting a high proliferative capacity of the founder cells. Very immature maphosphamide-resistant progenitors were detectable. The marked expansion in the circulating pool was predictable and reliable, allowing harvesting, after two or three leukaphereses, of sufficient haematopoietic progenitors for autologous bone-marrow reconstitution.
在20例非霍奇金淋巴瘤或乳腺癌患者中，高剂量环磷酰胺在白细胞快速恢复的最低点后时期，即中位第19天，使粒细胞-巨噬细胞（CFU-GM）和红系（BFU-E）集落形成细胞的峰值浓度增加约30倍，而更不成熟的多能祖细胞（CFU-Mix）增加更高（72倍）。输注重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）后，峰值浓度出现得更早（中位第15天）且进一步升高（CFU-GM、BFU-E和CFU-Mix数量分别增加159倍、116倍和283倍）。大多数CFU-GM不成熟，缺乏分化抗原CD15，并产生大的髓系集落，反映出原始细胞的高增殖能力。可检测到非常不成熟的耐马磷酰胺祖细胞。循环池中祖细胞的显著扩增是可预测且可靠的，在进行两到三次白细胞分离术后，能够采集到足够的造血祖细胞用于自体骨髓重建。
