Komissarova N V, Anokhin K V
P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow.
Neurosci Behav Physiol. 2008 Mar;38(3):289-96. doi: 10.1007/s11055-008-0041-z.
We report here studies on the effects of an imprinting procedure on cell proliferation in neonatal chicks in brain structures known to undergo plastic changes in imprinting. Proliferating cells were detected immunohistochemically on brain sections by incorporation of pre-training doses of 5-bromodeoxyuridine (BrdU) into DNA; numbers of new cells were counted in the intermediate medial mesopallium, the intermediate arcopallium, the medial part of the mesopallium and the nidopallium, the dorsocaudal nidopallium, the hippocampus, and the parahippocampal region 24 h and seven days after training. The intermediate medial mesopallium showed an increase in the number of BrdU-positive cells 24 h after training. However, at seven days post-training, the number of BrdU-containing cells decreased in the medial nidopallium and mesopallium, in the dorsocaudal nidopallium, and the right intermediate medial mesopallium. Thus, the imprinting procedure had differently directed transient and long-term influences on the genesis of new cells in the chick brain, inducing the appearance of a large number of cells in the parenchyma of the brain one day after training and decreases in the numbers of cells at later time points. This double effect may be associated with the fact that the imprinting procedure simultaneously initiates two brain processes involving the control of cell proliferation - one related to maturation of a species-specific functional system for tracking individuals of the same species and one related to remembering the characteristics of the actual parent.
我们在此报告关于印记程序对新生雏鸡大脑结构中细胞增殖影响的研究，这些大脑结构已知在印记过程中会发生可塑性变化。通过将训练前剂量的5-溴脱氧尿苷（BrdU）掺入DNA，在脑切片上用免疫组织化学方法检测增殖细胞；在训练后24小时和7天，对中间内侧中脑皮层、中间弓状皮层、中脑皮层内侧部分和巢皮层、背尾侧巢皮层、海马体以及海马旁区域的新细胞数量进行计数。训练后24小时，中间内侧中脑皮层的BrdU阳性细胞数量增加。然而，在训练后7天，内侧巢皮层和中脑皮层、背尾侧巢皮层以及右侧中间内侧中脑皮层中含BrdU的细胞数量减少。因此，印记程序对雏鸡大脑中新细胞的生成具有不同方向的短期和长期影响，在训练后一天诱导大脑实质中出现大量细胞，而在随后的时间点细胞数量减少。这种双重效应可能与以下事实有关，即印记程序同时启动了两个涉及细胞增殖控制的大脑过程——一个与追踪同一物种个体的物种特异性功能系统的成熟有关，另一个与记住实际亲本的特征有关。
