Watkins S
Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 14:Unit 14.2. doi: 10.1002/0471142727.mb1402s07.
This unit describes sample preparation and sectioning methods for frozen tissue. Sections of this type are used in a variety of light microscopic procedures including in situ hybridization, immunohistochemistry, and enzyme histochemistry.
本单元介绍了冷冻组织的样本制备和切片方法。这类切片用于多种光学显微镜检查程序,包括原位杂交、免疫组织化学和酶组织化学。