冷冻切片术

Cryosectioning.

作者信息

Son Alexander I, Sokolowski Katie, Zhou Renping

机构信息

Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.

出版信息

Methods Mol Biol. 2013;1018:301-11. doi: 10.1007/978-1-62703-444-9_28.

DOI:10.1007/978-1-62703-444-9_28

PMID:23681639

Abstract

Cryosectioning, the sectioning of frozen specimens, has been an important histological tool for more than a century and continues to be extensively utilized today. However, the ability to produce high-quality sections is often a difficult process requiring extensive patience and experience. In this chapter, we have detailed an effective method for the embedding, mounting, and sectioning of frozen tissues, as well as have provided suggestions in producing high-quality sections.

摘要

冷冻切片，即对冷冻标本进行切片，在一个多世纪以来一直是一种重要的组织学工具，并且至今仍被广泛使用。然而，制作高质量切片的能力往往是一个困难的过程，需要极大的耐心和经验。在本章中，我们详细介绍了一种冷冻组织包埋、贴片和切片的有效方法，并提供了制作高质量切片的建议。