College of Biosystems Engineering and Food Science, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China; Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States.
Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, United States.
Carbohydr Polym. 2021 May 15;260:117797. doi: 10.1016/j.carbpol.2021.117797. Epub 2021 Feb 14.
Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has resulted in a pandemic and continues to spread at an unprecedented rate around the world. Although a vaccine has recently been approved, there are currently few effective therapeutics to fight its associated disease in humans, COVID-19. SARS-CoV-2 and the related severe acute respiratory syndrome (SARS-CoV-1), and Middle East respiratory syndrome (MERS-CoV) result from zoonotic respiratory viruses that have bats as the primary host and an as yet unknown secondary host. While each of these viruses has different protein-based cell-surface receptors, each rely on the glycosaminoglycan, heparan sulfate as a co-receptor. In this study we compare, for the first time, differences and similarities in the structure of heparan sulfate in human and bat lungs. Furthermore, we show that the spike glycoprotein of COVID-19 binds 3.5 times stronger to human lung heparan sulfate than bat lung heparan sulfate.
严重急性呼吸综合征相关冠状病毒-2（SARS-CoV-2）已导致大流行，并继续以空前的速度在全球范围内传播。尽管最近已经批准了一种疫苗，但目前针对人类相关疾病 COVID-19，几乎没有有效的治疗方法。SARS-CoV-2 和相关的严重急性呼吸综合征（SARS-CoV-1）和中东呼吸综合征（MERS-CoV）是源自动物呼吸道病毒的人畜共患病，蝙蝠是其主要宿主，而目前尚不清楚的是其次要宿主。虽然这些病毒中的每一种都有不同的基于蛋白质的细胞表面受体，但它们都依赖糖胺聚糖肝素硫酸盐作为共受体。在这项研究中，我们首次比较了人类和蝙蝠肺中肝素硫酸盐的结构差异和相似之处。此外，我们还表明 COVID-19 的刺突糖蛋白与人类肺肝素硫酸盐的结合强度比蝙蝠肺肝素硫酸盐强 3.5 倍。
