Manzi A E
University of California San Diego, La Jolla, California, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 17:Unit17.17A. doi: 10.1002/0471142727.mb1717as32.
Selective cleavage of the oligosaccharide moiety of glycolipids for further structural analysis can be achieved by means of endoglycoceramidase (EGCase), an enzyme specific for the linkages between oligosaccharide and ceramide residues in glycolipids. The method described here can be used for analysis of glycolipids present in macroscopic amounts or for glycolipids that have been radiolabeled in their carbohydrate moiety.
通过内糖神经酰胺酶（EGCase）可以实现对糖脂寡糖部分的选择性切割，以便进行进一步的结构分析。EGCase是一种对糖脂中寡糖与神经酰胺残基之间的连接具有特异性的酶。这里描述的方法可用于分析大量存在的糖脂，或用于其碳水化合物部分已被放射性标记的糖脂。
