对糖缀合物中的碳水化合物进行直接化学分析。
Direct chemical analysis of glycoconjugates for carbohydrates.
作者信息
Manzi A, Esko J
机构信息
University of California San Diego, La Jolla, California, USA.
出版信息
Curr Protoc Mol Biol. 2001 May;Chapter 17:Unit17.9. doi: 10.1002/0471142727.mb1709s32.
PMID:18265165
Abstract
This unit presents protocols for detection of different sugars bound to glycoconjugates. The solution containing the carbohydrate material is treated with a specific reagent, generating a colored reaction product that can be detected spectrophotometrically. For each assay, the absorbance of the colored solution is proportional to the amount of sugar present in the glycoconjugate.
摘要
本单元介绍了检测与糖缀合物结合的不同糖类的方法。含有碳水化合物材料的溶液用特定试剂处理，生成可通过分光光度法检测的有色反应产物。对于每种测定，有色溶液的吸光度与糖缀合物中存在的糖量成正比。
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