用间苯二酚硫酸微量法比色测定中性糖
Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.
作者信息
Monsigny M, Petit C, Roche A C
机构信息
Laboratoire de Biochimie Cellulaire et Moléculaire des Glycoconjugués, Centre National de la Recherche Scientifique, Orleans, France.
出版信息
Anal Biochem. 1988 Dec;175(2):525-30. doi: 10.1016/0003-2697(88)90578-7.
A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.
本文描述了一种用于中性糖的微量比色测定法，其中中性糖在75%硫酸溶液存在下与间苯二酚反应。该测定法基于使用具有96个U形孔的微量滴定板，操作简单；它能够对含有1至100纳摩尔中性糖的小样本（20微升）进行准确测定，并且对于检测色谱柱流出物中的糖缀合物非常方便。
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