Conner D A
Harvard Medical School, Boston, Massachusetts, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 23:Unit 23.4. doi: 10.1002/0471142727.mb2304s52.
Building on the technology presented in earlier units of Chapter 23, this unit describes the basic method for the isolation of mouse embryonic (ES) stem cells. ES cells from wild-type mice may be used for the production of mouse mutants by homologous recombination and blastocyst-mediated transgenesis. ES cells from mutant mouse lines may be used in the analysis of mutant phenotypes.
基于第23章前面单元介绍的技术，本单元描述了分离小鼠胚胎（ES）干细胞的基本方法。野生型小鼠的ES细胞可用于通过同源重组和囊胚介导的转基因技术产生小鼠突变体。突变小鼠品系的ES细胞可用于分析突变表型。
